Immunohistochemical profile of cell cycle control, proliferation and differentiation proteins of the pulmonary atypical alveolar epithelium with COVID-19-induced diffuse alveolar damage as compared to pulmonary [lepidic-growth] adenocarcinoma

Aim. We aim to compare immunophenotypic characteritics of atypical epithelium (AE) with COVID-19-induced diffuse alveolar damage (DAD) and pulmonary lepidic-growth adenocarcinoma, accounting for cell cycle control, proliferation differentiation]. Methods. examined tissue specimens from twenty-four fatal cases acute respiratory syndrome confirmed by autopsy (Group 1) four adenocarcinoma 2). Perpendicular dimensions 10 nuclei were measured on the H&E slides, means their sums products (SPNM) calculated. have used p53, Ki67, p16, p63 antibodies immunohistochemical staining in each case. evaluate colour intensity, rate stained cells AE product these parameters. evaluated separately Nuclear cytoplasmic (couple) only (cyt) p16 expression. proliferative index at KI-67 slides. U-test Spearman rank correlation test statistical analysis. Results. Expression was higher group 1 (p=0.001), while more frequently expressed 2 (p=0.002). found no statistically significant differences (p>0.1) p53 Ki67 Group showed There negative between number days onset symptoms following variables: (r=–0.587, p=0.003); SPNM (r=–0.406, p=0.049). Conclusion. The present study has shown heterogeneity levels control expression, differentiation damage.